Polysome profiling and ribosome footprinting of Drosophila mature oocyte and activated egg
We use mRNA-seq in combination with polysome profiling to determine translational status for all mRNAs in Drosophila mature oocytes and activated eggs. Puromycin-treated lysates are used as a negative control in polysome profiling experiments. Additionally, we use ribosome footprinting to globally measure translational efficiency of mRNAs in wild type mature oocytes as well as wild type and png mutant activated eggs.
Provenance — who produced it, who reused it
Linked to 6 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Translation of CircRNAs 2017 · 1,871 cites
- mRNA poly(A)-tail changes specified by deadenylation broadly res... 2016 · 176 cites
- Genome-wide maps of ribosomal occupancy provide insights into ad... 2018 · 102 cites
- riboCIRC: a comprehensive database of translatable circRNAs 2021 · 82 cites
1 further paper cites this accession but reuse could not be confirmed.
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
No quantitative QC rubric exists for this data type yet, so it is deliberately left unscored — this is an honest "not applicable", not a poor rating.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently