Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE54460

GEO first seen 2014

RNAseq Analysis of Formalin-Fixed Paraffin-Embedded Prostate Cancer Tissues

Organism
Homo sapiens
Samples
106
Type
Expression profiling by high...
Submitted
2014-01-28

In an effort to identify biomarkers of recurrence, we have performed global RNA-sequencing on 106 formalin-fixed, paraffin-embedded (FFPE) prostatectomy samples from 100 patients at three independent sites, and identified a new set of biomarkers of biochemical recurrence composed of a 24-gene panel. We validated this 24-gene panel on an independent publicly available dataset of 140 patients and this new panel outperformed previously published markers based on cell proliferation gene sets in term...

Provenance — who produced it, who reused it

Linked to 30 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Carlos S Moreno
Reused by

27 further papers cite this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
106 / 106 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 302777015608 reported
total reads 3510303746 reported
n content pct 0.002 measured
pct q20 bases 97.7 measured
pct q30 bases 92.9 measured
gc content pct 51.5 measured
mean read length 50 measured
mean base quality 36.6 measured
adapter content pct 0 measured
duplication rate pct 89.22 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.9 measured ×1 100%
mean base quality 36.6 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 89.22 measured ×0.4 0%
QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0