Corpus 1,283 assessed · 1,184 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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GSE56638

GEO first seen 2015

Deep sequencing reveals cell-type specific patterns of single cell transcriptome variation

Organism
Mus musculus; Rattus norvegicus
Samples
137
Type
Expression profiling by high...
Submitted
2014-04-09

We present high quality deep read-depth single cell RNA sequencing for 91 cells from five mouse tissues and 18 cells from two rat tissues, along with 30 control samples of bulk RNA diluted to single-cell levels. We find that transcriptomes differ globally across tissues with regard to the number of genes expressed, the average expression patterns, and within cell-type variation patterns. We develop methods to filter genes for reliable quantification and to calibrate biological variation. All cel...

Provenance — who produced it, who reused it

Linked to 4 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Hannah DueckMugdha KhaladkarTae K KimJennifer M SpaethlingChantal FrancisSangita SureshStephen FisherPatrick SealeSheryl G BeckTamas BartfaiBernhard KuhnJim EberwineJunhyong Kim
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

metadata only · no data-level QC for this type

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

No quantitative QC rubric exists for this data type yet, so it is deliberately left unscored — this is an honest "not applicable", not a poor rating.

QC cost 22 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently