Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE59765

GEO first seen 2018

EGFR and MEK pathway signature RNA-Seq datasets

Organism
Homo sapiens
Samples
24
Type
Expression profiling by high...
Submitted
2014-07-25

EGFR and MEK pathways were activated alone or in combination in human mammary epithelial cells. We profiled the pathway gene expression signatures using RNA-Seq.

Provenance — who produced it, who reused it

Linked to 4 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Andrea BildW E Johnson
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (Homo sapiens). A grade, excellent for reuse. Exceptional base quality (Q30=97.7%, mean Q=38.3) combined with zero adapter contamination ensures highly reliable transcript quantification across the full dynamic range.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Files available
TXT
Metrics (value · how obtained)
n content pct 0.015 measured
pct q20 bases 99.2 measured
pct q30 bases 97.7 measured
gc content pct 50 measured
mean read length 49 measured
mean base quality 38.3 measured
adapter content pct 0 measured
duplication rate pct 27.55 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.7 measured ×1 100%
mean base quality 38.3 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 27.55 measured ×0.4 100%

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 92