Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE60106

GEO first seen 2014

Global loss of DNA methylation uncovers intronic enhancers in genes

Organism
Homo sapiens
Samples
11
Type
Methylation profiling by hig...
Submitted
2014-08-05

We used HCT116 colorectal cancer cells with and without mutations in DNA methyltransferases (resulting in a 95% reduction in global DNA methylation levels) to study the relationship between DNA methylation, histone modifications, and gene expression. (The double knockout cell line is called DKO1)

Provenance — who produced it, who reused it

Linked to 7 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Adam BlattlerHeather WittYu GuoCharles NicoletPeggy FarnhamFides LayPeter Jones
Reused by

3 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.007 measured
pct q20 bases 97 measured
pct q30 bases 93 measured
gc content pct 51 measured
mean read length 50 measured
mean base quality 36.5 measured
adapter content pct 0 measured
duplication rate pct 18.84 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93 measured ×1 100%
mean base quality 36.5 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 18.84 measured ×0.4 100%
QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0