Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE60361

GEO first seen 2016

Single-cell RNA-seq of mouse cerebral cortex

Organism
Mus musculus
Samples
3,005
Type
Expression profiling by high...
Submitted
2014-08-12

We have applied a recently developed, highly accurate and sensitive single-cell RNA-seq method (STRT/C1) to perform a molecular census of two regions of the mouse cerebral cortex: the somatosensory cortex and hippocampus CA1.

Provenance — who produced it, who reused it

Linked to 66 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Amit ZeiselAna B Muñoz ManchadoPeter LönnerbergSten Linnarsson
Reused by

46 further papers cite this accession but reuse could not be confirmed.

Deep data QC

76/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
3,492 / 3,005 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 342325477033 reported
total reads 6513937715 reported
n content pct 0 measured
pct q20 bases 98.1 measured
pct q30 bases 96.2 measured
gc content pct 42.9 measured
mean read length 52 measured
mean base quality 36.5 measured
adapter content pct 10.02 measured
duplication rate pct 84.32 measured
supplementary file types TXT, XLSX reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 76/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.2 measured ×1 100%
mean base quality 36.5 measured ×0.6 100%
adapter content pct 10.02 measured ×0.4 55%
duplication rate pct 84.32 measured ×0.4 0%
QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0