Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE60548

GEO first seen 2014

Rapid neurogenesis through transcriptional activation in human stem cell (RNA-Seq)

Organism
Homo sapiens
Samples
12
Type
Expression profiling by high...
Submitted
2014-08-20

Advances in cellular reprogramming and stem cell differentiation now enable ex vivo studies of human neuronal differentiation. However, it remains challenging to elucidate the underlying regulatory programs because differentiation protocols are laborious and often result in low neuron yields. Here, we overexpressed two murine Neurogenin transcription factors in human induced pluripotent stem cells, and obtained neurons with bipolar morphology in four days at greater than 90% purity. The high pur...

Provenance — who produced it, who reused it

Linked to 5 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Volker BuskampNathan Lewis
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

58/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.01 measured
pct q20 bases 88.8 measured
pct q30 bases 77.6 measured
gc content pct 48.9 measured
mean read length 101 measured
mean base quality 31.8 measured
adapter content pct 0.07 measured
duplication rate pct 48.63 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 58/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 77.6 measured ×1 38%
mean base quality 31.8 measured ×0.6 63%
adapter content pct 0.07 measured ×0.4 100%
duplication rate pct 48.63 measured ×0.4 59%
QC cost 50 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0