ChIP-seq profiling of loci bound by HIF1a in wild-type E12.5 hearts
Purpose: to identify genes directly bound by HIF1a in cardiomycoytes Methods: crosslinked chromatin isolated from E12.5 mouse hearts was precipitated using an anti-HIF1a antibody and processed for sequencing. A list of genes containing a HIF1a peak (Minimum score 3.5) was compared with RNA-seq results to identify genes that are bound by HIF1a and expressed in E12.5 hearts Results: this analysis identified 1016 genes expressed and bound by HIF1a in embryonic hearts
Provenance — who produced it, who reused it
Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- HIF1α Represses Cell Stress Pathways to Allow Proliferation of H... 2015 · 155 cites
- HIF1 mediates a switch in pyruvate kinase isoforms after myocard... 2018 · 76 cites
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
No quantitative QC rubric exists for this data type yet, so it is deliberately left unscored — this is an honest "not applicable", not a poor rating.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently