Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE63119

GEO first seen 2015

Multiplex-NGS for identification of differentially expressed miRNAs between colon cancer patients with and without metachronous metastases

Organism
Homo sapiens
Samples
50
Type
Non-coding RNA profiling by...
Submitted
2014-11-07

Purpose: The goal of this experiment was to identify differentially expressed miRNAs between colon cancer patients with and without metachronous metastases. Background: Colon cancer prognosis and treatment are currently based on a classification system still showing large heterogeneity in clinical outcome, especially in TNM-stages II-III. Prognostic biomarkers for metastasis risk are warranted, as development of distant recurrent disease mainly accounts for the high lethality rates of colon can...

Provenance — who produced it, who reused it

Linked to 5 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Peter J KuppenInès J Goossens-Beumer
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (Homo sapiens). B grade, marginal—use with caution. Extreme duplication (79.87%) suggests PCR overamplification or sample bias, which inflates expression variance and distorts DE calls; duplicate filtering or independent validation is essential before interpretation.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.013 measured
pct q20 bases 97.1 measured
pct q30 bases 91.9 measured
gc content pct 57.8 measured
mean read length 53 measured
mean base quality 36 measured
adapter content pct 0 measured
duplication rate pct 79.87 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.9 measured ×1 100%
mean base quality 36 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 79.87 measured ×0.4 0%

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 100