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Drop-Seq analysis of mixtures of human (HEK) and mouse (3T3) cells (1000/100 STAMPs) at 50 cells per microliter
A cell supsension containing an equal mix of HEK and 3T3 cells was used in Drop-Seq, a novel technology for high-throughput single cell mRNAseq. The cells were mixed at a final concentration of 50 cells per microliter.
Provenance — who produced it, who reused it
Linked to 11 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deposited / produced by
Evan Z Macosko
Reused by
- Fully-automated and ultra-fast cell-type identification using sp... 2022 · 783 cites
- An atlas of the aging lung mapped by single cell transcriptomics... 2019 · 670 cites
- A Single-Cell Transcriptomic Atlas of Human Neocortical Developm... 2019 · 567 cites
- An ultra high-throughput method for single-cell joint analysis o... 2019 · 380 cites
- dropEst: pipeline for accurate estimation of molecular counts in... 2018 · 212 cites
- The arginine methyltransferase PRMT7 promotes extravasation of m... 2022 · 128 cites
- Integrative analysis of cell state changes in lung fibrosis with... 2021 · 103 cites
- Systems-Level Properties of EGFR-RAS-ERK Signaling Amplify Local... 2020 · 88 cites
- High-throughput RNA sequencing of paraformaldehyde-fixed single... 2021 · 74 cites
2 further papers cite this accession but reuse could not be confirmed.
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
Data type / assay
bulk-RNA-seq
Organism
Homo sapiens; Mus musculus
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
2 / 2 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok
yes
reported
total bases
34856878000
reported
total reads
497955400
reported
supplementary file types
BAM, TXT
reported
QC cost
4 s compute
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently