RNA-seq of human fibroblasts during replicative senescence
Senescent human fibroblasts were compared to young proliferating fibroblasts. Five different cell lines were compared. Illumina sequencing (HiSeq2000) was applied to generate 50bp single-end reads. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Provenance — who produced it, who reused it
Linked to 12 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Transcriptomic alterations during ageing reflect the shift from... 2018 · 141 cites
- Genomic hallmarks and therapeutic implications of G0 cell cycle... 2023 · 74 cites
10 further papers cite this accession but reuse could not be confirmed.
Deep data QC
100/100 · AStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Large-scale human bulk-RNA-seq on Illumina HiSeq 2000 platform delivering 1.87 billion reads (93.5 Gb) with exceptional quality of 97.8% Q20 and 94.1% Q30. The 0.004% N-content indicates minimal sequencing errors, and GC composition near 50% reflects standard mammalian transcriptomes, supporting robust differential expression and isoform-level studies.
The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0