Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE64016

GEO first seen 2015

Single cell RNA-seq data of human hESCs to evaluate Oscope - a statistical pipeline for identifying oscillatory genes in unsynchronized single cell RNA-Seq

Organism
Homo sapiens
Samples
460
Type
Expression profiling by high...
Submitted
2014-12-09

Oscillatory gene expression is fundamental to mammalian development, but technologies to monitor expression oscillations are limited. We have developed a statistical approach called Oscope to identify and characterize the transcriptional dynamics of oscillating genes in single-cell RNA-seq data from an unsynchronized cell population. Applications to a number of data sets, include a single-cell RNA-seq data set of human embroyonic stem cells (hESCs), demonstrate advantages of the approach and als...

Provenance — who produced it, who reused it

Linked to 9 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Leng NingChu Li-Fang
Reused by

5 further papers cite this accession but reuse could not be confirmed.

Deep data QC

95/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 1.964 measured
pct q20 bases 96.8 measured
pct q30 bases 96 measured
gc content pct 45.5 measured
mean read length 51 measured
mean base quality 37.9 measured
adapter content pct 0.01 measured
duplication rate pct 43.82 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 95/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96 measured ×1 100%
mean base quality 37.9 measured ×0.6 100%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 43.82 measured ×0.4 69%
QC cost 39 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0