GSE65101

GEO first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

Sequencing the cap-snatching repertoire of H1N1 influenza provid... 2015 · 89 cites

Deep data QC

46/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Influenza A virus (H1N1) bulk RNA-seq contains 40 bp reads with moderate quality (78% Q30, 90.5% Q20) and elevated N-content (2.4%), reflecting the challenges of sequencing viral genomes with variable coverage. Despite these limitations, the dataset can yield insights into viral transcription patterns and host response signatures, though abundant-transcript analyses are preferable.

Data type / assay

bulk-RNA-seq

Organism

Influenza A virus (A/WSN/1933(H1N1))

Metrics (value · how obtained)

n content pct 2.404 measured

pct q20 bases 90.5 measured

pct q30 bases 78 measured

gc content pct 55.2 measured

mean read length 40 measured

mean base quality 31 measured

adapter content pct 0 measured

duplication rate pct 95.22 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 46/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78 measured ×1 40%

mean base quality 31 measured ×0.6 50%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 95.22 measured ×0.4 0%

QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0