Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE66961

GEO first seen 2015

Identification of in vivo DNA-binding mechanisms of Pax6 and reconstruction of Pax6-dependent gene regulatory networks during lens and forebrain development

Organism
Mus musculus
Samples
24
Type
Genome binding/occupancy pro...
Submitted
2015-03-16

The transcription factor Pax6 is comprised of the paired domain (PD) and homeodomain (HD). In the developing forebrain, Pax6 is expressed in ventricular zone precursor cells and in specific subpopulations of neurons; absence of Pax6 results in disrupted cell proliferation and cell fate specification. Pax6 also regulates the entire lens developmental program. To reconstruct Pax6-dependent gene regulatory networks (GRNs), ChIP-seq studies were performed using lens and forebrain chromatin from mice...

Provenance — who produced it, who reused it

Linked to 10 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Jian SunShira RockowitzQing XieRuth Ashery-PadanDeyou ZhengAles Cvekl
Reused by

6 further papers cite this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.001 measured
pct q20 bases 93.5 measured
pct q30 bases 85.1 measured
gc content pct 39.5 measured
mean read length 101 measured
mean base quality 34.1 measured
adapter content pct 0 measured
duplication rate pct 2.21 measured
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.1 measured ×1 76%
QC cost 33 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0