Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE67835

GEO first seen 2015

A survey of human brain transcriptome diversity at the single cell level

Organism
Homo sapiens
Samples
466
Type
Expression profiling by high...
Submitted
2015-04-14

We used single cell RNA sequencing on 466 cells to capture the cellular complexity of the adult and fetal human brain at a whole transcriptome level. Healthy adult temporal lobe tissue was obtained from epileptic patients during temporal lobectomy for medically refractory seizures. We were able to classify individual cells into all of the major neuronal, glial, and vascular cell types in the brain.

Provenance — who produced it, who reused it

Linked to 58 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Spyros DarmanisMartin EngeStephen R QuakeSteven A SloanBen A BarresYe ZhangChristine CanedaMelanie G Hayden GephartLawrence M Shuer
Reused by

36 further papers cite this accession but reuse could not be confirmed.

Deep data QC

86/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
466 / 466 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 294779103266 reported
total reads 1387000215 reported
n content pct 0.003 measured
pct q20 bases 92.5 measured
pct q30 bases 85.1 measured
gc content pct 36.8 measured
mean read length 75 measured
mean base quality 33.6 measured
adapter content pct 0.19 measured
duplication rate pct 36.9 measured
supplementary file types CSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 86/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.1 measured ×1 76%
mean base quality 33.6 measured ×0.6 93%
adapter content pct 0.19 measured ×0.4 100%
duplication rate pct 36.9 measured ×0.4 85%
QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0