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Enhancer activation during EGF response
We investigated the acetylation of H3K27, in normal condition and after stimulation of EGF and before or after the depletion of INTS11. Additionally, we examined transcription by sequencing the chromatin-bound fraction of RNA and by fractionating total RNA into polyadenylated and non-polyadenylated compartement.
Provenance — who produced it, who reused it
Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deposited / produced by
Alessandro GardiniFan LaiAnda ZhangRamin Shiekhattar
Reused by
- The Integrator Complex Attenuates Promoter-Proximal Transcriptio... 2019 · 239 cites
- The PP2A-Integrator-CDK9 axis fine-tunes transcription and can b... 2021 · 220 cites
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
Data type / assay
ChIP-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
30 / 30 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok
yes
reported
total bases
91196671066
reported
total reads
1238961693
reported
supplementary file types
BW
reported
QC cost
6 s compute
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently