Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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GSE70685

GEO first seen 2016

Musashi-2 attenuates AHR signalling to expand human haematopoietic stem cells

Organism
Homo sapiens
Samples
8
Type
Expression profiling by high...
Submitted
2015-07-09

A greater understanding of the molecular pathways that underpin the unique human hematopoietic stem and progenitor cell (HSPC) self-renewal program will improve strategies to expand these critical cell types for regenerative therapies. The post-transcriptional mechanisms guiding HSPC fate during ex vivo expansion have not been closely investigated. Using shRNA-mediated knockdown, we show that the RNA-binding protein (RBP) Musashi-2 (MSI2) is required for human HSPC self-renewal. Conversely, when...

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Brian T WilhelmKristin HopeStefan RentasNicholas HolzapfelMuluken S BelewGabriel PrattGary BaderVeronique VoisinGene Yeo
Reused by

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 2.368 measured
pct q20 bases 96 measured
pct q30 bases 93 measured
gc content pct 47.4 measured
mean read length 100 measured
mean base quality 36 measured
adapter content pct 0.01 measured
duplication rate pct 15.07 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93 measured ×1 100%
mean base quality 36 measured ×0.6 100%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 15.07 measured ×0.4 100%
QC cost 36 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0