Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

GSE72655

GEO first seen 2016

Insights into function and regulation of microRNAs by decoding degradation dynamics [4SU]

Organism
Mus musculus
Samples
24
Type
Non-coding RNA profiling by...
Submitted
2015-09-02

The regulation of the miRNA pool is one of the critical events in the definition of cell identity and behavior both in steady-state condition as well during cell transitions. While mechanisms controlling biogenesis have received much attention, little is known about how miRNA are turned over, due to the lack of proper approaches to analyze miRNA decay without globally perturbing gene transcription or miRNA biogenesis. To overcome such limitations we developed a “pulse-chase” approach for the ana...

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Heiko MullerMatteo J MarziFrancesco Nicassio
Reused by

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.182 measured
pct q20 bases 96.4 measured
pct q30 bases 93.5 measured
gc content pct 52.8 measured
mean read length 51 measured
mean base quality 37 measured
adapter content pct 0 measured
duplication rate pct 83.59 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.5 measured ×1 100%
mean base quality 37 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 83.59 measured ×0.4 0%
QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0