Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE74767

GEO first seen 2017

A developmental coordinate of the spectrum of pluripotency among mice, monkeys, and humans

Organism
Macaca fascicularis; Mus musculus
Samples
492
Type
Expression profiling by high...
Submitted
2015-11-06

The epiblast (EPI) is the origin of all somatic and germ cells in mammals, and of the spectrum of pluripotent stem cells (PSCs) in vitro. To explore the ontogeny of human/primate pluripotency, we performed comprehensive single-cell RNA sequencing for pre- and post-implantation EPI development in cynomolgus monkeys. Here we show that after specification in the blastocysts [embryonic day (E)7], cyEPI undergoes major transcriptome changes upon implantation. Thereafter, cyEPI, while generating gastr...

Provenance — who produced it, who reused it

Linked to 14 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Tomonori NakamuraIkuhiro OkamotoKotaro SasakiYukihiro YabutaChizuru IwataniHideaki TsuchiyaTakuya YamamotoMitinori Saitou
Reused by

5 further papers cite this accession but reuse could not be confirmed.

Deep data QC

45/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Macaca fascicularis
Metrics (value · how obtained)
n content pct 0 measured
pct q20 bases 78 measured
pct q30 bases 68 measured
gc content pct 0 measured
mean read length 51 measured
mean base quality 31.3 measured
adapter content pct 0 measured
duplication rate pct 35.36 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 45/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 68 measured ×1 0%
mean base quality 31.3 measured ×0.6 55%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 35.36 measured ×0.4 88%
QC cost 32 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0