Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE76118

GEO first seen 2016

Transcriptomic profiling maps anatomically patterned subpopulations among single embryonic cardiac cells [RNA-seq]

Organism
Mus musculus
Samples
3,241
Type
Expression profiling by high...
Submitted
2015-12-17

Embryonic gene expression intricately reflects anatomical context, developmental stage, and cell type. To address whether the precise spatial origins of cardiac cells can be deduced solely from their transcriptional profiles, we established a genome-wide expression database from 118, 949, and 1166 single murine heart cells at embryonic days (e)8.5, 9.5, and 10.5, respectively. We segregated these cells by type using unsupervised bioinformatic analysis and identified novel chamber-specific genes....

Provenance — who produced it, who reused it

Linked to 5 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Guang LiAdele XuSean Wu
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

80/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
3,241 / 3,241 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 1927173009890 reported
total reads 9540460445 reported
n content pct 0.005 measured
pct q20 bases 96.2 measured
pct q30 bases 87.9 measured
gc content pct 47.8 measured
mean read length 101 measured
mean base quality 34.8 measured
adapter content pct 18.91 measured
duplication rate pct 18.74 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 80/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 87.9 measured ×1 90%
mean base quality 34.8 measured ×0.6 100%
adapter content pct 18.91 measured ×0.4 6%
duplication rate pct 18.74 measured ×0.4 100%
QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0