Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE77314

GEO first seen 2016

RNA-seq of 50 paired hepatocellular carcinoma

Organism
Homo sapiens
Samples
100
Type
Expression profiling by high...
Submitted
2016-01-27

RNA-seq of Illumina sequencing of 50 paired normal and tumor samples

Provenance — who produced it, who reused it

Linked to 31 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Guojun HouGang LiuLei LiuWeiping Zhou
Reused by

27 further papers cite this accession but reuse could not be confirmed.

Deep data QC

70/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina Genome Analyzer
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
100 / 100 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 673915299600 reported
total reads 3369576498 reported
n content pct 0.023 measured
pct q20 bases 88.3 measured
pct q30 bases 79.6 measured
gc content pct 49.1 measured
mean read length 100 measured
mean base quality 32 measured
adapter content pct 0.15 measured
duplication rate pct 28.97 measured
supplementary file types XLSX reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 70/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 79.6 measured ×1 48%
mean base quality 32 measured ×0.6 67%
adapter content pct 0.15 measured ×0.4 100%
duplication rate pct 28.97 measured ×0.4 100%
QC cost 21 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0