Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE77509

GEO first seen 2017

RNA-seq analysis of hepatocellular carcinoma with portal vein tumor thrombosis

Organism
Homo sapiens
Samples
60
Type
Expression profiling by high...
Submitted
2016-02-02

Portal vein tumor thrombosis is a strong poor indication of HCC. Characterizing the molecular alterations of these metastatic HCCs is important for understanding the molecular mechanisms during HCC progression and metastasis. Previous genomic studies mainly focus on single molecular layer, such as gene expressions or exonic mutations. In this study, we systematically examined the copy number variation (CNV), DNA methylation, miRNA and transcriptome of matched adjacent normal tissues, primary tum...

Provenance — who produced it, who reused it

Linked to 22 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Gu JinChen Lei
Reused by

17 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
282 / 60 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 965274280200 reported
total reads 4826371401 reported
n content pct 0.005 measured
pct q20 bases 96 measured
pct q30 bases 90 measured
gc content pct 43.6 measured
mean read length 100 measured
mean base quality 35.8 measured
adapter content pct 0.25 measured
duplication rate pct 11.57 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90 measured ×1 100%
mean base quality 35.8 measured ×0.6 100%
adapter content pct 0.25 measured ×0.4 100%
duplication rate pct 11.57 measured ×0.4 100%
QC cost 28 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0