Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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GSE78040

GEO first seen 2016

Reversible methylation of m6Am in the 5′ cap controls mRNA stability

Organism
Homo sapiens; Mus musculus
Samples
28
Type
Expression profiling by high...
Submitted
2016-02-17

Internal bases in mRNA can be subjected to modifications that influence the fate of mRNA in cells. One of the most prevalent modified bases is found at the 5′ end of mRNA, at the first encoded nucleotide adjacent to the 7-methylguanosine cap. Here we show that this nucleotide, N6,2′-O-dimethyladenosine (m6Am), is a reversible modification that influences cellular mRNA fate. Using a transcriptome-wide map of m6Am we find that m6Am-initiated transcripts are markedly more stable than mRNAs that beg...

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Jan MauerSamie R JaffreyMegerditch Kiledjian
Reused by

Deep data QC

99/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.026 measured
pct q20 bases 94.8 measured
pct q30 bases 90.4 measured
gc content pct 50.2 measured
mean read length 101 measured
mean base quality 35.3 measured
adapter content pct 2.51 measured
duplication rate pct 31.69 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 99/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.4 measured ×1 100%
mean base quality 35.3 measured ×0.6 100%
adapter content pct 2.51 measured ×0.4 97%
duplication rate pct 31.69 measured ×0.4 96%
QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0