Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE78220

GEO first seen 2017

mRNA expressions in pre-treatment melanomas undergoing anti-PD-1 checkpoint inhibition therapy

Organism
Homo sapiens
Samples
28
Type
Expression profiling by high...
Submitted
2016-02-23

PD-1 immune checkpoint blockade provides significant clinical benefits for cancer patients. However, factors influencing innate sensitivity remain incompletely catalogued. We analyzed the somatic mutanomes and transcriptomes of pretreatment melanoma biopsies. Mutations in cell adhesion genes and the DNA repair gene BRCA2 were enriched in responding tumors, and a high mutational load associated with improved survival. Innately resistant tumors displayed frequent transcriptomic up-expression of ge...

Provenance — who produced it, who reused it

Linked to 142 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Willy HugoJesse M ZaretskyLu SunChunying SongBlanca Homet-MorenoSiwen Hu-LieskovanBeata Berent-MaozJia PangBartosz ChmielowskiGrace CherryElizabeth SejaShirley LomeliXiangju KongMark C KelleyJeffrey A SosmanDouglas B JohnsonAntoni RibasRoger S Lo
Reused by

63 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (human). Grade A quality (100/100) with clean measured metrics throughout. Q30 of 93.4% and low duplication (18.21%) both achieve perfect scores; minimal adapter content. Highly reusable for expression analysis.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Files available
XLSX
Metrics (value · how obtained)
n content pct 0.005 measured
pct q20 bases 97.5 measured
pct q30 bases 93.4 measured
gc content pct 51 measured
mean read length 100 measured
mean base quality 36.1 measured
adapter content pct 0.24 measured
duplication rate pct 18.21 measured
supplementary file types XLSX reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.4 measured ×1 100%
mean base quality 36.1 measured ×0.6 100%
adapter content pct 0.24 measured ×0.4 100%
duplication rate pct 18.21 measured ×0.4 100%

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 100