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Buffer composition affects ribosome footprint precision in Arabidopsis root and shoot
The goal of this study is to test ionic strength and buffering capacity in polysome extraction buffer on ribosome footprints in Arabidopsis root and shoot.
Provenance — who produced it, who reused it
Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deposited / produced by
Polly Y HsuPhilip N Benfey
Reused by
- Super-resolution ribosome profiling reveals unannotated translat... 2016 · 287 cites
- CircCode: A Powerful Tool for Identifying circRNA Coding Ability 2019 · 75 cites
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
Data type / assay
other
Organism
Arabidopsis thaliana
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
8 / 8 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok
yes
reported
total bases
4151423900
reported
total reads
83028478
reported
supplementary file types
TXT
reported
QC cost
6 s compute
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently