Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE81682

GEO first seen 2016

The transcriptional landscape of mouse blood stem/progenitor cell transitions at single cell resolution

Organism
Mus musculus
Samples
3,840
Type
Expression profiling by high...
Submitted
2016-05-20

Current gene-expression databases for the haematopoietic system provide information on gene expression profiles present in bulk populations. Although informative, these studies lack the resolution that can be gained at a single-cell level. In particular, population-average data assumes homogeneity within the population and may as such obscure the ability to detect the heterogeneity of decision-making processes in individual cells. Here we report 1656 single cell transcriptomes analysed by single...

Provenance — who produced it, who reused it

Linked to 33 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Sonia NestorowaNicola WilsonDavid KentFiona HameyEvangelia Diamanti
Reused by

23 further papers cite this accession but reuse could not be confirmed.

Deep data QC

32/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
3,840 / 3,840 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 1297575754250 reported
total reads 10380606034 reported
n content pct 0.007 measured
pct q20 bases 83.7 measured
pct q30 bases 76.5 measured
gc content pct 43.2 measured
mean read length 125 measured
mean base quality 32.5 measured
adapter content pct 25.36 measured
duplication rate pct 76.79 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 32/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 76.5 measured ×1 33%
mean base quality 32.5 measured ×0.6 75%
adapter content pct 25.36 measured ×0.4 0%
duplication rate pct 76.79 measured ×0.4 0%
QC cost 21 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0