GSE84534

GEO first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

A multifunctional AAV–CRISPR–Cas9 and its host response 2016 · 631 cites

Deep data QC

94/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

High-coverage mouse bulk RNA-seq from NextSeq 500 (337M reads, 96.7% Q30) with exceptional quality metrics; deep sampling enables detection of low-abundance transcripts and comprehensive isoform mapping suitable for systems-level mouse transcriptomics.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

72 / 18 runs

Completeness

100%

Metrics (value · how obtained)

checksum ok yes reported

total bases 50577758550 reported

total reads 337185057 reported

n content pct 0.006 measured

pct q20 bases 97.7 measured

pct q30 bases 96.7 measured

gc content pct 48.5 measured

mean read length 75 measured

mean base quality 35 measured

adapter content pct 0.05 measured

duplication rate pct 45.21 measured

supplementary file types XLS reported

How this grade was computed

Weighted mean of 4 scored metric(s) → 94/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.7 measured ×1 100%

mean base quality 35 measured ×0.6 100%

adapter content pct 0.05 measured ×0.4 100%

duplication rate pct 45.21 measured ×0.4 66%

QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0