GSE85075

GEO first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

Small RNA sequencing revealed dysregulated piRNAs in Alzheimer's... 2017 · 128 cites

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human bulk-RNA-seq with high base quality (Q30=94.6%, mean=34.6%) but extreme duplication (90.74%, scored 0/100). Duplication at this level reflects severe PCR amplification bias that will bias relative expression estimates. Not suitable for sensitive comparative studies.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Metrics (value · how obtained)

n content pct 0.001 measured

pct q20 bases 96.3 measured

pct q30 bases 94.6 measured

gc content pct 50.4 measured

mean read length 76 measured

mean base quality 34.6 measured

adapter content pct 0 measured

duplication rate pct 90.74 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.6 measured ×1 100%

mean base quality 34.6 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 90.74 measured ×0.4 0%

QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0