Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE85791

GEO

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

This is a human bulk RNA-seq dataset, and its QC reading is robust rather than provisional: every contributing metric was directly measured (evidence_strength=1), so the perfect 100/100 grade-A verdict rests on real FastQC-style measurements, not extrapolation, despite the incidental 429 fetch error on auxiliary metadata. The grade is driven chiefly by the highest-weighted metric, pct_q30_bases at 93.9% (paired with a mean base quality of Phred 36.4), meaning the overwhelming majority of bases clear the Q30 accuracy threshold — a strong signal that read-level base calls are trustworthy for variant calling and confident transcript quantification. Adapter contamination is low (1.79%), so minimal trimming overhead is expected before alignment. The one metric a reuser should eyeball is the 28.4% duplication rate; it did not penalize the score and is typical of RNA-seq libraries where highly expressed transcripts inflate duplicates, but if your downstream analysis is duplication-sensitive you should confirm it reflects biological expression rather than low library complexity.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.005 measured
pct q20 bases 98.1 measured
pct q30 bases 93.9 measured
gc content pct 50.3 measured
mean read length 100 measured
mean base quality 36.4 measured
adapter content pct 1.79 measured
duplication rate pct 28.36 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.9 measured ×1 100%
mean base quality 36.4 measured ×0.6 100%
adapter content pct 1.79 measured ×0.4 100%
duplication rate pct 28.36 measured ×0.4 100%
QC cost 22 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it mean score 68