Transcriptome profiling of 5 human adenocarcinoma cell lines
Purpose: The aim of this study is to compare the transcriptome profiles of a limited number of lung cancer cell lines with the intention of selecting the two most similar cell lines for a mixture experiment (GSE64098). Methods - Cell Culture: Five lung adenocarcinoma cell lines (H2228, NCI-H1975, HCC827, H838 and A549) from a range of passages (2-4) were grown on 2 separate occasions in RPMI media (Gibco) supplemented with Glutamax and 10\% fetal calf serum to a 70\% confluence. To replicate com...
Provenance — who produced it, who reused it
Linked to 11 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Heavy-tailed prior distributions for sequence count data: removi... 2018 · 2,246 cites
- A systematic evaluation of single-cell RNA-sequencing imputation... 2020 · 324 cites
- Comprehensive characterization of single-cell full-length isofor... 2021 · 226 cites
- Metacells untangle large and complex single-cell transcriptome n... 2022 · 86 cites
7 further papers cite this accession but reuse could not be confirmed.
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently