Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE87515

GEO first seen 2017

The m6A Demethylase ALKBH5 Maintains Tumorigenicity of Glioblastoma Stem-Like Cells by Sustaining FOXM1 Expression and Cell Proliferation Programing

Organism
Homo sapiens
Samples
8
Type
Expression profiling by high...
Submitted
2016-09-30

The dynamic and reversible N6-methyladenosine (m6A) RNA modification installed and erased by N6-methyltransferases and demethylases regulates gene expression and cell fate. Here, we show that the m6A demethylase ALKBH5 is highly expressed in glioblastoma stem-like cells (GSCs). Silencing ALKBH5 suppresses the proliferation of patient-derived GSCs in vitro and in vivo. Integrated transcriptome and m6A-seq analyses revealed altered expression of select ALKBH5 target genes, including FOXM1, a criti...

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Boxuan S ZhaoChuan He
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.019 measured
pct q20 bases 99.5 measured
pct q30 bases 98.3 measured
gc content pct 53.6 measured
mean read length 50 measured
mean base quality 39.8 measured
adapter content pct 0.02 measured
duplication rate pct 29.53 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 98.3 measured ×1 100%
mean base quality 39.8 measured ×0.6 100%
adapter content pct 0.02 measured ×0.4 100%
duplication rate pct 29.53 measured ×0.4 100%
QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0