Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE94660

GEO first seen 2019

RNA sequencing (RNA-SEQ) of 21 HBV-HCC patients with non-neoplastic liver and tumor tissues

Organism
Homo sapiens
Samples
42
Type
Expression profiling by high...
Submitted
2017-02-08

Purpose: Chronic Hepatitis B virus (HBV) infection leads to liver fibrosis which is a major risk factor in Hepatocellular carcinoma (HCC) and an independent risk factor of recurrence after HCC tumor resection. HBV genome can be inserted into human genome, and chronic inflammation may trigger somatic mutations. Several studies characterized HBV integration sites in HCC patients with regard to frequently occurring hotspots. However, how HBV integration and other genomic changes contribute to the r...

Provenance — who produced it, who reused it

Linked to 21 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Seungyeul YooQin WangJun ZhuSpiros P Hiotis
Reused by

18 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
42 / 42 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 349821158600 reported
total reads 1749105793 reported
n content pct 0 measured
pct q20 bases 98.1 measured
pct q30 bases 94.3 measured
gc content pct 46.9 measured
mean read length 100 measured
mean base quality 36.6 measured
adapter content pct 0.04 measured
duplication rate pct 21.6 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.3 measured ×1 100%
mean base quality 36.6 measured ×0.6 100%
adapter content pct 0.04 measured ×0.4 100%
duplication rate pct 21.6 measured ×0.4 100%
QC cost 20 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0