Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE96518

GEO first seen 2017

Transcriptomic and proteomic landscape of mitochondrial dysfunction reveals secondary coenzyme Q deficiency in mammals

Organism
Mus musculus
Samples
40
Type
Expression profiling by high...
Submitted
2017-03-12

Differential gene expression in mouse models of mitochondrial dysfunction

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Inge KuhlMaria MirandaIlian AtanassovIrina KuznetsovaYvonne HinzeArnaud MourierAleksandra FilipovskaNils-Goran Larsson
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

This is a bulk RNA-seq dataset from mouse, and on balance it is a solid, reusable resource that earned its B (85/100) on the strength of genuinely measured, high-quality base calls. The grade is driven up by excellent sequencing accuracy — 98.1% of bases at Q30 and a mean base quality of ~39.9, with zero detectable adapter contamination — meaning reads are clean and base-level errors are unlikely to confound expression estimates. The single factor pulling the score down is a duplication rate of 70.19% (scored just 11/100), which is high but not unusual for RNA-seq of highly expressed transcripts; for reuse it matters because it inflates apparent read depth and can bias quantification, so deduplication and library-complexity checks are advisable before differential-expression work. Because every contributing metric here was actually measured (evidence_strength=1), this reading is not provisional — the only gap is an upstream HTTP 429 fetch error on ancillary metadata, which does not affect the QC values reported.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.019 measured
pct q20 bases 99.4 measured
pct q30 bases 98.1 measured
gc content pct 41.9 measured
mean read length 75.5 measured
mean base quality 39.9 measured
adapter content pct 0 measured
duplication rate pct 70.19 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 98.1 measured ×1 100%
mean base quality 39.9 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 70.19 measured ×0.4 11%
QC cost 9 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 89