Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE97179

GEO first seen 2017

Single Cell Methylome Signatures Reveal Distinct Neuronal Populations and Regulatory Elements in the Mammalian Cortex

Organism
Homo sapiens; Mus musculus
Samples
6,172
Type
Methylation profiling by hig...
Submitted
2017-03-29

The mammalian brain contains diverse neuron cell types with distinct physiological, morphological and molecular characteristics. However, a comprehensive assessment of the epigenetically distinct neuronal classes is currently missing. Cytosine DNA methylation is a stable epigenetic mark that distinguishes neuron types and marks gene regulatory elements. We developed an efficient single-nucleus methylome sequencing approach that allows robust high-throughput neuron-type classification. We generat...

Provenance — who produced it, who reused it

Linked to 9 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Chongyuan LuoChristopher L KeownLaurie KuriharaJingtian ZhouYupeng HeJunhao LiRosa CastanonJacinta LuceroJoseph R NeryBrian BuiTimothy HarkinsEran A MukamelM M BehrensJoseph R Ecker
Reused by

4 further papers cite this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
methylation
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.02 measured
pct q20 bases 96.1 measured
pct q30 bases 90.9 measured
gc content pct 23.4 measured
mean read length 150.3 measured
mean base quality 37.8 measured
adapter content pct 0 measured
duplication rate pct 11.36 measured
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published methylation thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.9 measured ×1 100%
QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0