RNA-seq based transcriptomic profiling of the mouse small intestine response to lipopolysaccharide treatment
Purpose:Integrity of the intestinal mucosal barrier is important as a first line of defense against bacteria and endotoxin, we aim to characterize the activation response of intestinal mucosa to LPS exposure in a mouse model. Methods:Intestinal mRNA profiles of LPS challenged and control mice were generated by deep sequencing, in triplicate, using Illumina HiSeq 2000 Results: Using an optimized data analysis pipeline(HISAT2/HT-Seq/DESeq2), 714 differentially expression protein-coding and noncodi...
Provenance — who produced it, who reused it
Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- In Inflamed Intestinal Tissues and Epithelial Cells, Interleukin... 2018 · 182 cites
- Prostaglandin E <sub>2</sub> promotes intestinal inflammation vi... 2021 · 101 cites
1 further paper cites this accession but reuse could not be confirmed.
Deep data QC
86/100 · BStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0