GSE97375

GEO first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

Prostaglandin E2 is essential for efficacious skeletal muscle st... 2017 · 236 cites

Deep data QC

84/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mouse bulk-RNA-seq from HiSeq 2500 with moderate quality across dimensions: Q30=86.1%, mean quality=32.6%, and adapter content 3.92% all constrain the B-grade. Duplication control (25.05%) is good; suitable for reuse if moderate quality variation is acceptable.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

6 / 6 runs

Completeness

100%

Metrics (value · how obtained)

checksum ok yes reported

total bases 7600848464 reported

total reads 100011164 reported

n content pct 0.009 measured

pct q20 bases 89.4 measured

pct q30 bases 86.1 measured

gc content pct 47.3 measured

mean read length 76 measured

mean base quality 32.6 measured

adapter content pct 3.92 measured

duplication rate pct 25.05 measured

supplementary file types TXT reported

How this grade was computed

Weighted mean of 4 scored metric(s) → 84/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 86.1 measured ×1 81%

mean base quality 32.6 measured ×0.6 77%

adapter content pct 3.92 measured ×0.4 89%

duplication rate pct 25.05 measured ×0.4 100%

QC cost 11 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0