Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE97955

GEO first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

Deep data QC

77/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Mouse bulk-RNA-seq from NextSeq 500 with moderate base quality but extremely high duplication (64.56%). The duplication rate—scored only 23/100—indicates severe PCR amplification bias that will distort expression quantification. Not recommended for reuse without specialized deduplication or digital PCR.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
80 / 80 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 29061440532 reported
total reads 418447163 reported
n content pct 0.001 measured
pct q20 bases 91.1 measured
pct q30 bases 87.5 measured
gc content pct 42.4 measured
mean read length 64.5 measured
mean base quality 32.9 measured
adapter content pct 0 measured
duplication rate pct 64.56 measured
supplementary file types XLSX reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 77/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 87.5 measured ×1 88%
mean base quality 32.9 measured ×0.6 82%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 64.56 measured ×0.4 23%
QC cost 11 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0