Targeted degradation of CTCF decouples local insulation of chromosome domains from genomic compartmentalization
The molecular mechanisms underlying folding of mammalian chromosomes remain poorly understood. The transcription factor CTCF is a candidate regulator of chromosomal structure. Using the auxin-inducible degron system in mouse embryonic stem cells, we show that CTCF is absolutely and dose-dependently required for looping between CTCF target sites and insulation of topologically associating domains (TADs). Restoring CTCF reinstates proper architecture on altered chromosomes, indicating a powerful i...
Provenance — who produced it, who reused it
Linked to 16 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Heterochromatin drives compartmentalization of inverted and conv... 2019 · 730 cites
- Promoter-proximal CTCF binding promotes distal enhancer-dependen... 2021 · 299 cites
- Distinct Classes of Chromatin Loops Revealed by Deletion of an R... 2019 · 275 cites
- Molecular basis of CTCF binding polarity in genome folding 2020 · 210 cites
- Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in E... 2020 · 205 cites
- Cohesin is required for long-range enhancer action at the Shh lo... 2022 · 157 cites
- Loop stacking organizes genome folding from TADs to chromosomes 2023 · 121 cites
- Orphan CpG islands amplify poised enhancer regulatory activity a... 2021 · 117 cites
- Distinct properties and functions of CTCF revealed by a rapidly... 2021 · 98 cites
- CTCF-dependent chromatin boundaries formed by asymmetric nucleos... 2019 · 74 cites
6 further papers cite this accession but reuse could not be confirmed.
Deep data QC
insufficient data to scoreStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0