Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE99074

GEO first seen 2019

Transcriptomic analysis of purified human cortical microglia reveals age-associated changes

Organism
Homo sapiens; Mus musculus
Samples
71
Type
Expression profiling by high...
Submitted
2017-05-18

Purpose: Microglia are essential for central nervous system (CNS) homeostasis and innate neuroimmune function, and play important roles in neurodegeneration and brain aging. Here, we present gene expression profiles of purified microglia isolated at autopsy from the parietal cortex of 39 human subjects with intact cognition. We identified an age-associated gene signature in human microglia that was enriched for genes involved in cell adhesion, axonal guidance, cell surface receptor expression, a...

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Thais F GalatroInge R HoltmanAntonio M LerarioIlia D VainchteinNieske BrowerPaula SolaMariana VerasTulio PereiraRenata LeiteThomas MöllerPaul D WesMari C SogayarJon D LamanWilfred denDunnenCarlos A PasqualucciSueli M Oba-ShinjoErik W BoddekeSuely N MarieBart J Eggen
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.006 measured
pct q20 bases 98.6 measured
pct q30 bases 96.3 measured
gc content pct 48.5 measured
mean read length 101 measured
mean base quality 37 measured
adapter content pct 1.61 measured
duplication rate pct 11.36 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.3 measured ×1 100%
mean base quality 37 measured ×0.6 100%
adapter content pct 1.61 measured ×0.4 100%
duplication rate pct 11.36 measured ×0.4 100%
QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0