Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE99533

GEO

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

82/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bacterial bulk RNA-seq from S. pyogenes; B-grade quality suitable for basic downstream analysis. Q30 base accuracy (83.9%) and duplication rate (43%) are the primary limiting factors—acceptable for gene-level quantification but may complicate isoform-level analysis.

Data type / assay
bulk-RNA-seq
Organism
Streptococcus pyogenes
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
4 / 4 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 46153506500 reported
total reads 231525701 reported
n content pct 0.281 measured
pct q20 bases 93.7 measured
pct q30 bases 83.9 measured
gc content pct 43.4 measured
mean read length 100 measured
mean base quality 34 measured
adapter content pct 0.1 measured
duplication rate pct 43.03 measured
supplementary file types CSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 82/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.9 measured ×1 70%
mean base quality 34 measured ×0.6 100%
adapter content pct 0.1 measured ×0.4 100%
duplication rate pct 43.03 measured ×0.4 71%
QC cost 11 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 flagged mean score 57