Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE99701

GEO first seen 2017

Transcriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-Seq

Organism
Mus musculus
Samples
262
Type
Expression profiling by high...
Submitted
2017-06-05

Purpose: Transport processes in the renal collecting duct are responsible for precise regulation of blood pressure and body fluid composition. The collecting duct is composed of at least three cell types, type A intercalated cells (A-IC), type B intercalated cells (B-IC) and principal cells (PC). To identify the genes that are selectively expressed in each cell type, including cell-surface receptors, transcription factors, transporters and secreted proteins, we used cell surface markers necessa...

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Lihe ChenJae Wook LeeChung-Lin ChouAnil V NairMaria A BattistoneTeodor G PăunescuMaria MerkulovaSylvie BretonJill W VerlanderSusan M WallBrown DennisMaurice B BurgMark A Knepper
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.031 measured
pct q20 bases 99.1 measured
pct q30 bases 97.2 measured
gc content pct 44.9 measured
mean read length 50 measured
mean base quality 39.5 measured
adapter content pct 0.01 measured
duplication rate pct 69.34 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.2 measured ×1 100%
mean base quality 39.5 measured ×0.6 100%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 69.34 measured ×0.4 13%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0