Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE99915

GEO first seen 2018

Single-cell mapping of lineage and identity in direct reprogramming

Organism
Mus musculus
Samples
28
Type
Expression profiling by high...
Submitted
2017-06-12

Direct lineage reprogramming involves the conversion of cellular identity. Single-cell technologies are useful for deconstructing the considerable heterogeneity that emerges during lineage conversion. However, lineage relationships are typically lost during cell processing, complicating trajectory reconstruction. Here we present ‘CellTagging’, a combinatorial cell-indexing methodology that enables parallel capture of clonal history and cell identity, in which sequential rounds of cell labelling...

Provenance — who produced it, who reused it

Linked to 5 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Samantha A MorrisBrent A Biddy
Reused by

3 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.047 measured
pct q20 bases 97.5 measured
pct q30 bases 96 measured
gc content pct 26.1 measured
mean read length 50 measured
mean base quality 38.2 measured
adapter content pct 1.94 measured
duplication rate pct 21.87 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96 measured ×1 100%
mean base quality 38.2 measured ×0.6 100%
adapter content pct 1.94 measured ×0.4 100%
duplication rate pct 21.87 measured ×0.4 100%
QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0