Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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GSM1015161

GEO first seen 2017

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

81/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Multi-organism bulk RNA-seq using Illumina HiSeq 2000 across diverse vertebrates (Xenopus tropicalis, Gallus gallus, Mus musculus, Tetraodon nigroviridis, Anolis carolinensis) with exceptionally high base quality (100% Q20/Q30). The ~1.37 billion short reads and 202 billion bases enable comparative transcriptomics across phylogenetically distant species. This dataset is well-suited for studying conserved versus lineage-specific gene expression patterns, though users should verify organism-specific biological variation isn't confounded with technical batch effects.

Data type / assay
bulk-RNA-seq
Organism
Xenopus tropicalis; Gallus gallus; Mus musculus; Tetraodon nigroviridis; Anolis carolinensis
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
21 / 21 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 202466139086 reported
total reads 1370901180 reported
n content pct 0.002 measured
pct q20 bases 100 measured
pct q30 bases 100 measured
gc content pct 53.3 measured
mean read length 72 measured
mean base quality 30 measured
adapter content pct 4.25 measured
duplication rate pct 23.74 measured
supplementary file types BW, TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 81/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%
mean base quality 30 measured ×0.6 33%
adapter content pct 4.25 measured ×0.4 88%
duplication rate pct 23.74 measured ×0.4 100%
QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0