GSM531976

GEO first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

17/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This human bulk-RNA-seq contains 35bp short reads with notably lower quality (82.3% Q20, 57.8% Q30) compared to modern standards, platform unspecified. The high error rate and substantial N-content (0.11%) raise concerns about data reliability for expression quantification; verification and preprocessing with quality filtering is essential.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Metrics (value · how obtained)

n content pct 0.11 measured

pct q20 bases 82.3 measured

pct q30 bases 57.8 measured

gc content pct 47.3 measured

mean read length 35 measured

mean base quality 26.3 measured

adapter content pct 0 measured

duplication rate pct 87.01 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 17/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 57.8 measured ×1 0%

mean base quality 26.3 measured ×0.6 0%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 87.01 measured ×0.4 0%

QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0