GSM721698

GEO first seen 2013

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

26/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This Homo sapiens bulk RNA-seq duplicates problematic metrics from a companion library—very poor base quality (Q30=39.3%, Q20=66%), high N-content (1.436%), and short 36 bp reads—indicating systematic preparation or sequencing issues that severely limit transcript quantification accuracy; only suitable for presence/absence transcript screening if quality filtering is applied stringently.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Metrics (value · how obtained)

n content pct 1.436 measured

pct q20 bases 66 measured

pct q30 bases 39.3 measured

gc content pct 56.8 measured

mean read length 36 measured

mean base quality 21.4 measured

adapter content pct 0.04 measured

duplication rate pct 49.8 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 26/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 39.3 measured ×1 0%

mean base quality 21.4 measured ×0.6 0%

adapter content pct 0.04 measured ×0.4 100%

duplication rate pct 49.8 measured ×0.4 56%

QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0