GSM747476

GEO first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

50/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This Homo sapiens bulk RNA-seq exhibits high per-base accuracy (Q30=82.5%, Q20=89.7%) across 76 bp reads, but carries substantial adapter contamination (29.03%) that will inflate mapping artifacts and inflate isoform abundance estimates—trimming adapters before alignment is mandatory for reliable downstream analysis.

Data type / assay

bulk-RNA-seq

Organism

Homo sapiens

Metrics (value · how obtained)

n content pct 0.011 measured

pct q20 bases 89.7 measured

pct q30 bases 82.5 measured

gc content pct 43.6 measured

mean read length 76 measured

mean base quality 31.9 measured

adapter content pct 29.03 measured

duplication rate pct 55.45 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 50/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.5 measured ×1 63%

mean base quality 31.9 measured ×0.6 65%

adapter content pct 29.03 measured ×0.4 0%

duplication rate pct 55.45 measured ×0.4 43%

QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0