GSM850909

GEO first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

28/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

This Mus musculus bulk RNA-seq combines suboptimal base quality (Q30=67.9%, Q20=72.3%) with short 36 bp reads and moderate N-content (0.51%), limiting sensitivity for low-abundance transcript detection—suitable primarily for measuring highly-expressed genes and detecting large fold-changes, though read quality necessitates conservative alignment parameters.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Metrics (value · how obtained)

n content pct 0.51 measured

pct q20 bases 72.3 measured

pct q30 bases 67.9 measured

gc content pct 46.5 measured

mean read length 36 measured

mean base quality 24.8 measured

adapter content pct 0.01 measured

duplication rate pct 44.2 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 28/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 67.9 measured ×1 0%

mean base quality 24.8 measured ×0.6 0%

adapter content pct 0.01 measured ×0.4 100%

duplication rate pct 44.2 measured ×0.4 68%

QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0