Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJDB7829

BioProject first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

98/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Spumella sp. NIES-1846 bulk RNA-seq via Illumina HiSeq 2500 with 22.4 million reads (4.5 billion bases) and outstanding quality (98.6% Q20, 96.9% Q30). The 56.2% GC reflects this heterotrophic flagellate's genomic composition. This dataset supports transcriptome profiling of this poorly-characterized protist and may answer questions about nutrient-sensing and grazing-induced gene expression changes.

Data type / assay
bulk-RNA-seq
Organism
Spumella sp. NIES-1846
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 4527692438 reported
total reads 22414319 reported
n content pct 0 measured
pct q20 bases 98.6 measured
pct q30 bases 96.9 measured
gc content pct 56.2 measured
mean read length 101 measured
mean base quality 37 measured
adapter content pct 0.38 measured
duplication rate pct 34.26 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 98/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.9 measured ×1 100%
mean base quality 37 measured ×0.6 100%
adapter content pct 0.38 measured ×0.4 100%
duplication rate pct 34.26 measured ×0.4 91%
QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0