Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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PRJEB10595

BioProject first seen 2016

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

69/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Mus musculus amplicon sequencing via 454 GS FLX Titanium with 956,716 reads and 516 million bases, read type unknown (low confidence) typical of 454 metadata gaps. The 94.8% Q20 and 56.5% GC are reasonable; longer 454 reads may offer advantages for certain targeted loci. Users should reconcile 454 chemistry limitations with their downstream analysis pipeline and verify marker-gene target.

Data type / assay
amplicon
Organism
Mus musculus
Instrument
454 GS FLX Titanium
Platform
LS454
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
3 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 516069414 reported
total reads 956716 reported
n content pct 0.01 measured
pct q20 bases 94.8 measured
pct q30 bases 80.7 measured
gc content pct 56.5 measured
mean read length 503.8 measured
mean base quality 35.1 measured
adapter content pct 0 measured
duplication rate pct 93.16 measured
mean target coverage 9.6 extrapolated
How this grade was computed
Weighted mean of 2 scored metric(s) → 69/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 80.7 measured ×1 54%
adapter content pct 0 measured ×0.5 100%
QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0