Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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PRJEB11448

BioProject first seen 2015

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

95/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This Illumina HiSeq 2500 bulk RNA-seq dataset from Homo sapiens delivers 127 million reads and 23 billion bases with exceptional quality (99.3% Q20, 97.2% Q30), providing sensitive transcriptome profiling across a wide dynamic range of expression levels. The high fidelity enables accurate quantification of isoform structure, splicing variants, and lowly-expressed transcripts without extensive trimming. Researchers can confidently apply this for gene expression quantification, differential expression testing, and pathway inference, though the fixed 100–125 bp read length may limit de novo assembly and complex structural-variant discovery.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), BAM/CRAM (aligned)
N numbers (samples, groups)
12 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 22919903280 reported
total reads 127332796 reported
n content pct 0 measured
pct q20 bases 99.3 measured
pct q30 bases 97.2 measured
gc content pct 47.1 measured
mean read length 90 measured
mean base quality 37.7 measured
adapter content pct 0 measured
duplication rate pct 44.8 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 95/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.2 measured ×1 100%
mean base quality 37.7 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 44.8 measured ×0.4 67%
QC cost 14 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0